Detection of Human RLF by Western Blot and Immunoprecipitation. Samples:  Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells.  Antibodies: Affinity purified rabbit anti-RLF antibody A303-207A used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate.  Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).