Detection of Human NO66 by Western Blot and Immunoprecipitation. Samples:  Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells.  Antibodies: Affinity purified rabbit anti-NO66 antibody A303-198A used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate.  NO66 was also immunoprecipitated by rabbit anti-NO66 antibody A303-199A, which recognizes a downstream epitope.   Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).