Detection of Human SENP1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SENP1 antibody A302-928A used for IP at 3 ug/mg lysate. SENP1 was also immunoprecipitated by rabbit anti-SENP1 antibody A302-927A, which recognizes an upstream epitope. For blotting immunoprecipitated SENP1, A302-927A was used at 0.4 ug/ml,. Detection: Chemiluminescence with an exposure time of 30 seconds.