Detection of Human F as by Western Blot and Immunoprecipitation. Samples:  Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells.   Antibodies: Affinity purified rabbit anti-F as antibody A302-858A used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.  Fas was also immunoprecipitated by rabbit anti-F as antibody BL10340, which recognizes an upstream epitope.  Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).