Detection of Human and Mouse RelB by Western Blot (h&m) and Immunoprecipitation (h).  Samples: Whole cell lysate from Jurkat (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug) and mouse NIH3T3 (M; 50?g) cells.  Antibodies: Affinity purified rabbit anti-RelB antibody A302-183A used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate.  RelB was also immunoprecipitated by rabbit anti-RelB antibody A302-182A, which recognizes an upstream epitope.  Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).