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Detection of Human ZEB1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells.  Antibodies: Affinity purified rabbit anti-ZEB1 antibody A301-921A used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.  ZEB1 was also immunoprecipitated by rabbit anti-ZEB1 antibody A301-922A, which recognizes a downstream epitope.  Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).