Detection of Human SOX2 by Western Blot and Immunoprecipitation.  Samples: Whole cell lysate from H520 (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells.  Antibodies: Affinity purified rabbit anti-SOX2 antibody A301-739A used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.  SOX2 was also immunoprecipitated by rabbit anti-SOX2 antibodies A301-740A and A301-741A, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).