Detection of Human ERCC5/XPG by Western Blot of Immunoprecipitates.  Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.  Antibodies: Affinity purified rabbit anti-ERCC5/XPG antibody A301-485A used for IP at 3 ug/mg lysate.  ERCC5/XPG was also immunoprecipitated by rabbit anti-ERCC5/XPG antibody A301-484A, which recognizes an upstream epitope.  For blotting immunoprecipitated ERCC5/XPG, A301-485A was used at 1 ug/ml.  Detection: Chemiluminescence with an exposure time of 30 seconds.