Detection of Human PARP1  by Immunohistochemistry and  Immunofluorescence.  Sample: FFPE sections of human breast carcinoma.  Antibody: Affinity purified rabbit anti-PARP1  (Cat. No. A301-376A Lot1) used at a dilution of 1:200 (1 ug/ml) and 1:80 (2.5 ug/ml).  Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated (Cat. No. A120-601D4) used at a dilution of 1:100