Detection of Human and Mouse PPM1B by WB (h&m) and Immunoprecipitation.  Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells.  Antibodies: Affinity purified rabbit anti-PPM1B antibody A300-887A used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B).  PPM1B was also immunoprecipitated by rabbit anti-PPM1B antibody A300-886A, which recognizes an upstream epitope.   Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).