Detection of Human DDX18 by Western Blot and Immunoprecipitation.  Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug for WB) cells.  Antibodies: Affinity purified rabbit anti-DDX18 antibody BL3018 (Cat. No. A300-636A) used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and for IP at 3 ug/mg lysate.  DDX18 was also immunoprecipitated using rabbit anti-DDX18 antibody BL3015 (Cat. No. A300-635A) at 3 ug/mg lysate.    Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).