Detection of Human CAP-G2 by Western Blot and Immunoprecipitation.  Samples:  Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells.  Antibodies: Affinity purified rabbit anti-CAP-G2 antibody A300-605A used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.  CAP-G2 was also immunoprecipitated by rabbit anti-CAP-G2 antibody BL2946, which binds an upstream epitope, and CAP-G2 was co-immunoprecipitated by rabbit anti-CAP-D3 antibody A300-604A.    Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 minutes (B).