Detection of Human Aurora B by Immunofluorescence. Samples: Asynchronous HeLa cells.  Antibody: Affinity purified rabbit anti-Aurora B antibody BL1075 (Cat. No. A300-431A) used at 0.5 ug/ml in A and at 2 ug/ml in B.  Detection: Secondary antibodies were anti-Rabbit IgG conjugated to FITC in A and conjugated to Texas Red in B.