Detection of Human and Mouse PHD1 by Western Blot. Samples: Recombinant epitope-tagged PHD1, PHD2 and PHD3 (10 ng/lane) or whole cell lysate from HeLa (human) or MEF (mouse) cells.  Antibody: Affinity purified rabbit anti-PHD1 (Cat. No. A300-326A) used at 1 ug/ml.  Detection: Chemiluminescence.