Detection of Human and Mouse Phospho-XRCC1-S485/T488 by Western Blot.  Samples: Whole cell lysate (50 ug) from asynchronously growing HeLa (H), 293T (T) and mouse NIH-3T3 (M) cells.  Antibodies: Affinity purified rabbit anti-XRCC1-S485/T488 antibody A300-231A (lot A300-231A-2) used for WB at 0.1 ug/ml.  The binding of the antibody was blocked by pre-incubation with the phospho-peptide but was not blocked by pre-incubation with the non-phosphorylated peptide.  Detection: Chemiluminescence with an exposure time of 30 seconds.