Western blot analysis of extracts from rat brain, using Piezo1 antibody.

DF12083 at 1/100 staining Human brain cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22!aC. An HRP conjugated goat anti-rabbit antibody was used as the secondary