Western blot analysis of p38 MAPK phosphorylation expression in Jurkat whole cell lysates.

AF4001 at 1/200 staining human kidney tissue sections by IHC-P.

AF4001 staining 293T treated with UV 30min by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25!aC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37!aC. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod