Western blot analysis of AMPK alpha phosphorylation expression in Heatshock treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Phospho-AMPK alpha (Thr172) Antibody for IHC in human colon.

af3423 staining C2C12 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37!aC. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37!aC. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibod

AF3423 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25!aC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37!aC. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod