MYC-tag polyclonal antibody (Cat # PAB10345) detects ~60 kDa amino terminal linked MYC-tagged recombinant protein by western blot (arrowhead).
Lane 1 contains ~35 ug of lysate from control 293T cells.
Lane 2 contains ~35 ug of lysate from 293T cells overexpressing an N-terminal linked recombinant protein.
Amino terminal linked MYC recombinant protein was the gift of Brian Conti, University of North Carolina, Chapel Hill, NC.