BALB/c spleen cells were activated by incubation for 72 hours with LPS. The cells were then harvested and stained with Cd86 monoclonal antibody, clone GL1 (FITC) (Cat # MAB5751). A wide gate was set to include small, medium and large cells, following which the cells were analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).