Western blot analysis of lysate from SH-SY5Y cell line, using Park2 Antibody (N-term) (Cat. #AP6402a). AP6402a was diluted at 1:1000 at each lane. A goat anti-rabbit (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Parkin Antibody (N-term) (Cat. #AP6402a) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of Parkin Antibody (N-term)(Cat#AP6402a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Parkin Antibody (N-term) (AP6402a)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Parkin Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.