STING M284 variant (human recombinant)

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• 構造式
• 製品情報
• 製品詳細
• 文献

価格

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構造式

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製品情報

STING M284 variant (human recombinant) contains amino acids 138-379 of the wild-type variant (R232) with a methionine substituted for arginine at position 284. Stimulator of interferon genes (STING) is a component of the innate immune response that binds to cyclic dinucleotides, which are bacterial second messengers, leading to activation of NF-κB and transcription of immunomodulatory genes, including type I interferon (IFN). The R232 variant of STING is the most common variant in the human population, found at a frequency of 57.9% in the 1000 Genome Project. The SNP variant H232 is found at a 13.7% frequency. The R284M mutation in STING is associated with constitutive activation of downstream signaling. It increases the propensity of STING to dimerize and associate with the kinase TBK1, enhancing the ability of STING to activate IRF3 and NF-κB and induce a type I IFN response. However, the R284M mutation occurs outside of the dimerization region between positions 153-177, so rather than a direct effect on dimerization, it is predicted to promote or inhibit binding of a cellular factor that stabilizes or impairs STING dimerization.

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製品詳細

name	STING M284 variant (human recombinant)
Synonyms	Endoplasmic Reticulum Interferon Stimulator ERIS hSTING MITA Stimulator of Interferon Genes Protein TMEM173 Transmembrane Protein 173
Purity	≥80% estimated by SDS-PAGE
Source	N-terminal His-tagged human recombinant protein purified from E. coli
Amino Acids	138-379 (N-terminal truncation)
Molecular Weight	28.8 kDa
Formulation	50 mM HEPES pH 8.0, 150 mM NaCl, 10% glycerol
UniProt Accession №	Q86WV6
Storage	-80°C
Shipping	Dry ice in continental US; may vary elsewhere
Stability	≥ 1 year

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文献

1. Sun, L., Wu, J., Du, F., et al. Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway. Science 339(6121), 786-791 (2013).

2. Wu, J., Sun, L., Chen, X., et al. Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA. Science 339(6121), 826-830 (2013).

3. Konno, H., Konno, K., and Barber, G.N. Cyclic dinucleotides trigger ULK1 (ATG1) phosphorylation of STING to prevent sustained innate immune signaling. Cell 155(3), 688-698 (2013).

4. Burdette, D.L., Monroe, K.M., Sotelo-Troha, K., et al. STING is a direct innate immune sensor of cyclic-di-GMP. Nature 478(7370), 515-518 (2011).

5. Yi, G., Brendel, V.P., Shu, C., et al. Single nucleotide polymorphisms of human STING can affect innate immune response to cyclic dinucleotides. PLoS One 8(10), e77846 (2013).

6. Tang, E.D., and Wang, C.-Y. Single amino acid change in STING leads to constitutive active signaling. PLoS One 10(3), e0120090 (2015).

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