STING R224 variant (human recombinant)

• 価格
• 構造式
• 製品情報
• 製品詳細
• 文献

価格

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構造式

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製品情報

STING R224 variant (human recombinant) contains amino acids 138-379 of the wild-type variant (R232) with lysine 224 substituted with arginine. Stimulator of interferon genes (STING) is a component of the innate immune response that binds to cyclic dinucleotides, which are bacterial second messengers, leading to activation of NF-κB and transcription of immunomodulatory genes, including type I interferon (IFN). The R232 variant of STING is the most common variant in the human population, found at a frequency of 57.9% in the 1000 Genome Project. The SNP variant H232 is found at a 13.7% frequency. The K224R mutation prevents ubiquitination of STING at position K224, a process which is essential for efficient cytosolic DNA-mediated signaling. Therefore, this mutation disrupts optimal STING trafficking, which inhibits TBK1-mediated IRF3 activation but not NF-κB activation.

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製品詳細

name	STING R224 variant (human recombinant)
Synonyms	Endoplasmic Reticulum Interferon Stimulator Stimulator of Interferon Genes TMEM173
Purity	≥75% estimated by SDS-PAGE
Source	N-terminally Histidine-tagged human STING R232, K224R mutant purified from E. coli
Amino Acids	138-379 (N-terminal truncation)
Molecular Weight	28.8 kDa
Formulation	50 mM HEPES, pH 8.0, 150 mM sodium chloride, and 10% glycerol
Storage	-80°C
Shipping	Dry ice in continental US; may vary elsewhere
Stability	≥ 1 year

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文献

1. Sun, L., Wu, J., Du, F., et al. Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway. Science 339(6121), 786-791 (2013).

2. Wu, J., Sun, L., Chen, X., et al. Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA. Science 339(6121), 826-830 (2013).

3. Konno, H., Konno, K., and Barber, G.N. Cyclic dinucleotides trigger ULK1 (ATG1) phosphorylation of STING to prevent sustained innate immune signaling. Cell 155(3), 688-698 (2013).

4. Burdette, D.L., Monroe, K.M., Sotelo-Troha, K., et al. STING is a direct innate immune sensor of cyclic-di-GMP. Nature 478(7370), 515-518 (2011).

5. Yi, G., Brendel, V.P., Shu, C., et al. Single nucleotide polymorphisms of human STING can affect innate immune response to cyclic dinucleotides. PLoS One 8(10), e77846 (2013).

6. Ni, G., Konno, H., and Barber, G.N. Ubiquitination of STING at lysine 224 controls IRF3 activation. Sci. Immunol. 2(11), eaah7119 (2017).

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