抗Human Prostatic Acid Phosphatase/ACPP抗体(Anti-Human Prostatic Acid Phosphatase/ACPP antibody)

掲載日情報:2019/10/21 現在Webページ番号:194851

Human Prostatic Acid Phosphatase/ACPPに対する抗体(Anti-Human Prostatic Acid Phosphatase/ACPP )です。
本製品は研究用です。研究用以外には使用できません。

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[在庫・価格 :2024年03月29日 00時00分現在]

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納期 文献数
Anti-Human Prostatic Acid Phosphatase/ACPP Affinity Purified PAb
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 1
説明文
別名:acid phosphatase
Genbank No: 55
Protein Accession No: P15309
別包装品 別包装品あり
法規制等
保存条件 法規備考
抗原種 免疫動物 Sheep クラス IgG 標識 Unlabeled
交差性 Human 適用 ELISA,IHC,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
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Anti-Human Prostatic Acid Phosphatase/ACPP Affinity Purified PAb
2~3週間 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
説明文
※受注発注品。形状:溶液または凍結乾燥
別名:acid phosphatase
Genbank No: 55
Protein Accession No: P15309
別包装品 別包装品あり
法規制等
保存条件 法規備考
抗原種 免疫動物 Sheep クラス IgG 標識 Unlabeled
交差性 Human 適用 ELISA,IHC,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事
関連記事

[在庫・価格 :2024年03月29日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Human Prostatic Acid Phosphatase/ACPP Affinity Purified PAb

文献数: 1

説明文 別名:acid phosphatase
Genbank No: 55
Protein Accession No: P15309
別包装品 別包装品あり
法規制等
保存条件 法規備考
抗原種 免疫動物 Sheep
交差性 Human 適用 ELISA,IHC,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事
関連記事

Anti-Human Prostatic Acid Phosphatase/ACPP Affinity Purified PAb

文献数: 0

説明文 ※受注発注品。形状:溶液または凍結乾燥
別名:acid phosphatase
Genbank No: 55
Protein Accession No: P15309
別包装品 別包装品あり
法規制等
保存条件 法規備考
抗原種 免疫動物 Sheep
交差性 Human 適用 ELISA,IHC,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事
関連記事



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Product Details

Species ReactivityHuman
LabelUnconjugated
ImmunogenMouse myeloma cell line NS0-derived recombinant human Prostatic Acid Phosphatase/ACPPLys33-Gln379Accession # P15309
SourcePolyclonal Sheep IgG
PurificationAntigen Affinity-purified
SpecificityDetects human Prostatic Acid Phosphatase/ACPP in direct ELISAs and Western blots.


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Applications and Data

 Recommended
Concentration
Sample
Western Blot1 µg/mLSee below
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Prostatic Acid Phosphatase/ACPP Monoclonal Antibody (Catalog # MAB62401).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Prostatic Acid Phosphatase/ACPP DuoSet ELISA Kit (Catalog # DY6240-05) for convenient development of a sandwich ELISA.

Immunohistochemistry5-15 µg/mLSee below


Western Blot
Detection of Human Prostatic Acid Phosphatase/ACPP by Western Blot.
Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF Membrane was probed with 1 µg/mL of Human Prostatic Acid Phosphatase/ACPP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6240) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Prostatic Acid Phosphatase/ACPP at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Immunohistochemistry
Prostatic Acid Phosphatase/ACPP in Human Prostate.
Prostatic Acid Phosphatase/ACPP was detected in immersion fixed paraffin-embedded sections of human prostate using Human Prostatic Acid Phosphatase/ACPP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6240) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hemotoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
ELISA
Human Prostatic Acid Phosphatase/ACPP ELISA Standard Curve.
Recombinant Human Prostatic Acid Phosphatase/ACPP protein was serially diluted 2-fold and captured by Mouse Anti-Human Prostatic Acid Phosphatase/ACPP Monoclonal Antibody (Catalog # MAB62401) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Prostatic Acid Phosphatase/ACPP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6240) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).


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Related Product & Information

Entrez Gene IDs55 (Human); 563181 (Mouse); 56780 (Rat)
BackgroundProstatic Acid Phosphatase/ACPP
background_contentBackground:
Prostatic Acid Phosphatase/ACPP
ACPP (Acid phosphatase, prostate; also PAP and ACP3) is a 48-52 kDa glycoprotein member of the histidine acid phosphatase family of enzymes. It exists as a 95-100 kDa nondisulfide-linked homodimer that hydrolyzes phosphate esters under low pH to generate free phosphate. ACPP is expressed by prostate epithelium and pain-detecting spinal cord neurons. In the spinal cord, ACPP dephosphorylates AMP. This generates adenosine which acts as a strong analgesic agent. Mature human ACPP is 354 amino acids (aa) in length (aa 33-386). It contains one histidine phosphatase domain (aa 34-332), plus a nucleophile acceptor site at His44, and a proton donor site at Asp290. There are two potential alternative splice variants. One shows a deletion of aa 153-185, while another is transmembrane (previously called TMPase) and shows a 38 aa substitution for the C-terminal seven amino acids. Over aa 33-379, human ACPP shares 84% aa identity with mouse ACPP.


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. Up-regulated Ectonucleotidases in Fas-Associated Death Domain Protein- and Receptor-Interacting Protein Kinase 1-Deficient Jurkat Leukemia Cells Counteract Extracellular ATP/AMP Accumulation via Pannexin-1 Channels during Chemotherapeutic Drug-Induced Apoptosis
    Authors: AM Boyd-Tress, GS Lane, GR Dubyak
    Mol. Pharmacol., 2017;92(1):30-47.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB



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