抗CD40抗体 | Anti-CD40 antibody

• 特長
• 価格
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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製品情報

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, ELISA
Reactivity	Mouse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	0.1-0.2 μg/ml
ELISA	0.5-1.0 μg/mL

Not tested in other applications.

Calculated MW	32 kDa. ( Note )
Specificity/Sensitivity	Shows <1% cross-reactivity with recombinant human CD40.

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PROPERTIES

Form	Liquid
Buffer	PBS
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Mouse
Immunogen	recombinant mouse CD40 extracellualr domain expressed in NSO cells.
Purification	Purified by affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	CD40 antigen , AI326936 , Bp50 , GP39 , HIGM1 , IGM , IMD3 , T-BAM , TRAP , Tnfrsf5 , p50
Background	CD40 is a transmembrane glycoprotein mainly expressed on the surface of B cells and is also expressed on monocytes, dendritic cells, endothelial cells, and epithelial cells. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the low affinity nerve growth factor (NGF) receptor and CD95/Fas. CD40 ligand (CD40L, CD154, gp39, TRAM) belongs to the TNF gene family and is expressed more widely than activated CD4+ T cells. Following interaction with CD40L, CD40 mediates a number of major immunoregulatory functions, is central to the control of thymus-dependent humoral immunity and may be critical in the development of cell-mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphoma and to induce the death of transformed cells of mesenchymal or epithelial origin. In their resting state up to 50% of B cells may express CD40L in their cytoplasm, but not on the surface and this cytoplasmic CD40L is readily released as a soluble receptor. The proportion of expressing cells and the amount of CD40L expressed is increased by signaling through immunoglobulin (Ig) or CD38. The signal transduction pathways triggered through CD40 have not yet been fully delineated. Early biochemical events include activation of Ras, of Lyn and Syk protein tyrosine kinases, phosphorylation and activation of phospholipase C2 and phosphoinositide 3 kinase, and induction of mitogen-activated protein kinases. Activation processes seem to be initiated by association of the CD40 receptor with signaling molecules of the TRAF family.
Database	・ Gene ID: 21939 Cd40 ・ UniProt: P27512 Cd40

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