抗ROCK1抗体 | Anti-ROCK1 antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

Lysates prepared from MDCK cells left untreated (1, 3) or treated with staurosporine (2) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ROCK1 (GTX10549) cleavage-site specific antibody (1, 2) or a ROCK1 pan antibody (3) in a 3% BSA-TBST buffer for two hours at room temperature. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected. The data show that ROCK1 [1113/1114] CSSA detected the 130 kDa cleaved form of ROCK but not the full length. This antibody did not detect the 130 kDa cleaved form of ROCK upon inhibition of caspase activity with the pan caspase inhibitor Ac-VAD-CHO (data not shown).

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Human, Mouse, Dog

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent

Not tested in other applications.

Calculated MW	158 kDa. ( Note )
Positive Control	MDCK cells treated with staurosporine

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PROPERTIES

Form	Liquid
Buffer	PBS pH 7.3 (without Mg2+ and Ca2+) containing 50% glycerol, 1.0mg/ml BSA (IgG, protease free). and 0.05% sodium azide.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Synthetic peptide (Human).
Purification	Immunogen affinity purified Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a spanning peptide corresponding to the cleavage-site to remove antibody that is reactive with non-cleavage-site specific ROCK1. T
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Rho associated coiled-coil containing protein kinase 1 , P160ROCK , ROCK-I
Cellular Localization	Cytoplasm,Cytoplasm, cytoskeleton,Cell membrane
Background	ROCK1, a 160 kDa small-GTP-effector protein, is a serine/threonine kinase involved in the induction of focal adhesions and stress fibers, and can play a major role in vascular smooth muscle contraction, malignant cell transformation, tumor invasion and metastasis. ROCK1 is identified as a substrate for caspases and is cleaved by caspase 3 at a conserved DETD1113/G sequence which removes its carboxy-terminal inhibitory domain, resulting in a constitutively active 130 kDa ROCK1 kinase. Proteolytic activation of ROCK1 by caspase is critical for its role in regulating the actin cytoskeleton and Myosin Light Chain (MLC) phosphorylation. Increased phosphorylation of MLC is important for membrane blebbing, a cellular event that occurs at the onset of apoptosis.
Database	・ Gene ID: 6093 ROCK1 ・ UniProt: Q13464 ROCK1

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