抗PHD2 [AT21E9]抗体 | Anti-PHD2 [AT21E9] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	HeLa cells were stained with monoclonal anti-EGLN1 antibody (Green). Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue).
	Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EGLN1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	AT21E9
Isotype	IgG2b
Application	WB, ICC/IF, ELISA
Reactivity	Human

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APPLICATION

Application Note

We recommend the following starting dilutions:
Western blot and Immunofluorescence analysis: Use at 1:500 ~ 1000. Recommended starting dilution is 1:500.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW	46 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Recombinant EGLN1 (271-426aa) purified from E. coli
Purification	By protein-G affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Egl-9 Family Hypoxia Inducible Factor 1 , C1Orf12 , Ecyt3 , Halah , Hif-Ph2 , Hifph2 , Hph-2 , Hph2 , Phd2 , Sm20 , Zmynd6 , Egln1
Background	PHD1 (Egln2), PHD-2 (Egln1), and PHD3 (Egln3) are members of the Egln family of proline hydroxylases. EGLN family, which is known to be cellular oxygen sensor, initiates the first step of HIF degradation through the VHL/ubiquitin pathway. PHD1 and PHD2 can hydroxylate both proline 402 and proline 564, but PHD3 can only hydroxylate proline 564. In addition to HIF, PHD enzymes have also has been shown to catalyze the hydroxylation of RNA polymerase subunits and myogenin.
Database	・ Gene ID: 54583 ・ UniProt: Q9GZT9

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