抗VEGF Receptor 2 (phospho Tyr1054/Tyr1059)抗体 | Anti-VEGF Receptor 2 (phospho Tyr1054/Tyr1059) antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of whole cell extracts (30 μg lysate) of HUVEC (Lane 1), treated with 25ng/mL VEGF for 5 minutes (lane 2), MDA-MB-231 (lane 3), and MCF7 (Lane 4) using GTX25473 VEGF Receptor 2 (phospho Tyr1054/Tyr1059) antibody. Dilution : 1:1000
	ICC/IF analysis of HUVEC cell treated with 100ng of VEGF for 30 minutes using GTX25473 VEGF Receptor 2 (phospho Tyr1054/Tyr1059) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.Green : Primary antibodyBlue : NucleiRed : ActinFixation : 4% paraformaldehydePermeabilization : 0.1% Triton X-100 for 10 minutes
	WB analysis of PAE cells transfected with a chimeric receptor unstimulated (Lane 1, 2) or stimulated with 40ng/mL CSF-1 for 10 minutes (Lane 3-8) using GTX25473 VEGF Receptor 2 (phospho Tyr1054/Tyr1059) antibody.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, FACS
Reactivity	Human, Mouse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000
ICC/IF	1:250
FACS	1:20

Not tested in other applications.

Calculated MW	152 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS pH 7.3, 1mg/ml BSA, 50% glycerol, 0.05% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human VEGFR2 that contains tyrosines 1054 and 1059. The sequence is conserved among multiple species including mouse and rat.
Purification	Purified by antigen-affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	kinase insert domain receptor , CD309 , FLK1 , VEGFR , VEGFR2
Cellular Localization	Cell junction,Endoplasmic reticulum,Cytoplasm,Nucleus,Cytoplasmic vesicle
Background	Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009]
Database	・ Gene ID: 3791 KDR ・ UniProt: P35968 KDR

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