R&D Systems now offers a Mouse Chitinase 3-like 3/ECF-L Quantikine ELISA Kit (Catalog # MC3L30 ). Chitinase 3-like 3 (CHI3L3), also known as eosinophil chemotactic factor-lymphocyte (ECF-L) and Ym1, is a secreted glycoprotein that shares homology with chitinase proteins, but lacks enzymatic activity. It is primarily produced by alveolar and peritoneal macrophages during inflammation, and is considered to be a marker for alternatively activated macrophages. CHI3L3 binds heparin and G1cN oligomers, and has been proposed to promote adhesive interactions between cells by upregulating LFA-1 and ICAM-1. Like other mammalian chitinase-like proteins, CHI3L3 is highly expressed under Th2-driven inflammatory conditions and may play a role in wound healing and asthma. The Mouse Chitinase 3-like 3/ECF-L Quantikine ELISA Kit is designed to detect Chitinase 3-like 3 in cell culture supernates, cell lysates, serum, and plasma.

Analysis of Chitinase 3-like 3 Levels in Cell Lysates using the Quantikine ELISA Kit. Aliquots of cell lysates prepared from homogenized tissues from the indicated mouse organs were assayed using the Mouse Chitinase 3-like 3/ECF-L Quantikine ELISA Kit (Catalog # MC3L30).