R&D Systems now offers Human Adiponectin/Acrp30 (Catalog # NFADIP) and Human HGF (Catalog # NFHGF) Biotinylated Fluorokine® Kits. These kits are designed for the detection of either Adiponectin receptor- or HGF receptor-expressing cells by flow cytometry. The Fluorokine Kits use either Biotinylated Recombinant Human Adiponectin or Biotinylated Recombinant Human HGF, along with avidin-fluorescein to label cells expressing Adiponectin or HGF receptors, respectively. Fluorescent cells are subsequently detected by flow cytometry. Both kits also include an appropriate blocking antibody and a negative control protein biotinylated to the same degree as the protein of interest to allow the specificity of the staining to be assessed.

Detection of Adiponectin Receptor-expressing Cells using the Human Adiponectin/Acrp30 Biotinylated Fluorokine Kit. A. The Human Adiponectin/Acrp30 Biotinylated Fluorokine Kit (Catalog #NFADIP) was used to assess Adiponectin receptor positive MDA-MB-231 human breast adenocarcinoma cells by flow cytometry. Cells before (open histogram) and after (filled histogram) labeling with the reagents provided in the kit are shown. B. The specificity of the Human Adiponectin/Acrp30 Biotinylated Fluorokine Kit was demonstrated by staining cells in the absence (filled histogram) or in the presence (open histogram) of the human Adiponectin blocking antibody provided in the kit.