R&D Systems is introducing the Proteome Profiler 96 Antibody Array, a new 96-well microplate-based platform for multi-analyte profiling. Three Proteome Profiler 96 Phospho-RTK Antibody Arrays (Catalog #ARZ001, ARZ002, ARZ003) are now available. Each consists of microplate wells pre-spotted with a carefully selected panel of capture antibodies. The assay utilizes the classic two-site sandwich immunoassay technique and a suitable camera system to detect the relative levels of up to 16 phosphorylated receptor tyrosine kinases (RTKs) in a single sample.

Features

• 96-well microplate-based format
• Up to 1536 data points in a single plate
• Requires small samples sizes (50-100 µL)
• Short assay time (3.5 hours)
• Amenable to automation
• Free analysis software automatically detects spots
• Utilizes chemiluminescence and common camera systems

Detection of PDGF-BB-induced RTK Phosphorylation using the Proteome Profiler 96 Phospho-RTK Antibody Array 1. A. The Proteome Profiler 96 Human Phospho-RTK Antibody Array 1 (Catalog #ARZ001) was used to simultaneously assess the phosphorylation of 16 different receptor tyrosine kinases (RTKs) in lysates from CCD1070Sk human foreskin fibroblasts that were either untreated or treated with recombinant human PDGF-BB (Catalog #220-BB) for five minutes. RS = Reference Spot. B. Histogram profiles for select proteins were generated by quantifying the mean spot pixel densities from individual antibody spots using analytical software.