R&D Systems now offers a human Dkk-1 Quantikine ELISA Kit (Catalog # DKK100). Dickkopf related protein 1 (Dkk-1) is a member of the Dkk family of secreted proteins. Dkk-1 acts as an antagonist of the canonical Wnt signaling pathway by binding to the Frizzled co-receptors LRP-5 and -6. Dkk-1 antagonizes Wnt-7a signaling during limb and retina development, and in developing neurons, keratinocytes, and hair follicles. Postnatally, the balance between Wnt signaling and Dkk-1 inhibition is critical for bone homeostasis. The human Dkk-1 Quantikine ELISA is designed to measure the levels of Dkk-1 in cell culture supernatants, serum, and plasma.

Assessment of Dkk-1 Levels in Cell Culture Supernatants using the Quantikine ELISA Kit. Aliquots of cell culture supernatants removed from human umbilical vein endothelial cells (HUVEC), A431 human epidermoid carcinoma cells, human epithelial keratinocytes (NHEK), and PC3 human prostate adenocarcinoma cells, unstimulated or stimulated with TNF-alpha, were assayed using the human Dkk-1 Quantikine ELISA Kit (Catalog #DKK100).