R&D Systems now offers a mouse IFN-gamma/IL-17 Dual Color ELISpot Kit (Catalog # ELD5007 ). This complete kit is designed to simultaneous detect IFN-gamma and IL-17-secreting cells at the single cell level. ELISpot assays are highly reproducible and sensitive and can be used to measure responses with frequencies below 1 in 100,000 cells. Additionally, ELISpot assays do not require prior cell expansion and are suitable for high-throughput analysis using only small volumes of primary cells.

Detection of IFN-gamma and IL-17 Secretion in Mouse Splenocytes. Mouse splenocytes were stimulated with PMA/Ca2+ ionomycin. IFN-gamma (blue spots) and IL-17 (red spots) secretion was detected using the mouse IFN-gamma/IL-17 Dual Color ELISpot Kit (Catalog # ELD5007).