組織および培養細胞分離／分散溶液 Accutase

: イノベーティブセルテクノロジーズ / Innovative Cell Technologies, Inc.

[掲載日情報：2008/07/15 現在]

接着細胞の剥離や，組織から初代培養細胞を分離，分散させるのに適した調製済み溶液です。

特長

プロテアーゼ活性とコラゲナーゼ活性が最大限に効果を発揮するように組み合わせてあります。
穏やかかつ迅速に作用するので，高い細胞生存率を示します。
哺乳動物細胞や微生物由来の物質は含んでいません。

・Testing: Accutase has been extensively tested in research and biopharmaceutical laboratories.

・Cell Lines: Accutase has been shown effective on: fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma cells, and Sf9 insect cells.

・Application: Accutase performs exceptionally well in detaching cells for: analysis of cell surface markers, virus growth assay, quiescence assays by serum starvation, transformation assays by oncogene transfection, neural crest cell migration assays, cell proliferation, cell haptotaxsis, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry.

・Formulation: Accutase has both protease and collagenolytic activity. Each lot of Accutase is individually formulated to meet quality control standards for effective cell detachment. Accutase is supplied in Dulbecco's PBS containing O.5mM EDTA·4Na and phenol red.

・Availability: Accutase is available in ready-to-use 100ml bottles & 500 ml bottles. ・The Accutase Advantage
Compare Accutase with your current method of cells detachiment:

Accutase	Others
YES	NO	Easy to use, cost effective
YES	NO	Effective for most cell culture applications
YES	NO	Gentle to cells
YES	NO	Tissue dissociation for primary cell culture
YES	NO	Non-prokaryote
YES	NO	Non-mammalian
YES	NO	No neutralization required
YES	NO	Effective at removing cells from SmartPlastic

・Cell Detachment Results
組織および培養細胞分離／分散溶液 Accutase

CELL DETACHMENT: Human MG63 fibrosarcoma cells cultured on tissue culture treated dishes in DMEM + 10% FBS were treated with ACCUTASE. Treatment results in rapid cell detachment, a single cell suspension, and high viability. ACCUTASE is gentle on cells; viability was 97 ± 3% even after 45 minutes in ACCUTASE.

・Accutase allows a greater recovery rate of frozen hESCs
組織および培養細胞分離／分散溶液 Accutase

hESC cultured in Invitrogen's Stempro hESC Serum and Freeder Free Media (SFM) were harvested either with Accutase or with Collagenase. Harvested cells were frozen down respectively in the same condition. After 2 month of Storage in LN2 , vials were thawed and plated respectively. After 1 day of recovery, phase images of the recovered cells, total cell number and viable cell number were obtained from respective group using a Countess automated cell counter.