Products Categories

BioDynamics Laboratory Inc.(株式会社バイオダイナミクス研究所)
バイオダイナミクス / BioDynamics Laboratory Inc.(株式会社バイオダイナミクス研究所)
Web Page No:
[Date:November 14 2017]

Next-Generation RIPA Buffer for High Efficient Membrane Protein Extraction ULTRARIPA kit for Lipid Raft

RIPA buffer is one of the most useful buffers for protein extraction. RIPA buffer maintains most native structures of proteins and the extracted proteins can be applied to various applications. However, RIPA buffer is not sufficient to extract membrane proteins and membrane-associated proteins concentrated in lipid raft.

Lipid raft is a highly specialized microdomain on the lipid bilayer which contains specialized lipids, cholesterol and functional proteins. These lipid rafts are also called “Detergent Resistant Membrane (DRM)”, as lipid raft-enriched proteins are usually insoluble by mild detergent buffers such as 1% Triton X-100 and RIPA buffer. Consequently, it was difficult to analyze functions of lipid raft-enriched proteins extracted with RIPA buffer.

BioDynamics Laboratory's newly developed product:the ULTRARIPA kit, can efficiently and rapidly extract membrane proteins or membrane-associated proteins enriched in lipid rafts, with little effect on protein structures and functions.

ULTRARIPA kit includes a totally new buffer not containing protein denaturing detergents, but can extract the DRM which was difficult to extract by conventional RIPA buffer. ULTRARIPA kit helps to analyze various biological assays of the proteins in lipid raft.

Lipid raft and Detergent Resistant Membrane (DRM)

Features of ULTRARIPA kit

  • Extract membrane / membrane associated proteins enriched in lipid rafts, with little effect on protein structures and functions.
  • Easy and Simple Procedure : Only a centrifuge is required
  • Only two components: A buffer and B buffer

Overview of Procedure

Kit components

A buffer (RIPA Buffer) : 100 mL
B buffer : 10 mL

ULTRARIPA kit for Lipid Raft component
Procedure of ULTRARIPA kit

Overview of advantages of ULTRARIPA kit

Protein Extraction Buffer Protein Extraction Protein Structure Protein Function Application
Cytosolic Membrane
Non-lipid raft Lipid raft
> 1% SDS buffer SDS-PAGE
RIPA buffer Enzymatic assay,
Immunoprecipitation,
SDS-PAGE,etc
ULTRARIPA kit

Protein extraction efficiency of ULTRARIPA kit

Extraction of RIPA-insoluble proteins by ULTRARIPA kit Extraction of RIPA-insoluble proteins such as Caveolin-1 by ULTRARIPA kit

Extraction of RIPA-insoluble proteins by ULTRARIPA kit

Left : Quantification of extracted total proteins by BCA protein assay. ULTRARIPA kit could constantly extract over 70% of RIPA-insoluble proteins from the mouse brain tissue.

Right : Western blotting of lipid raft markers. Some lipid raft markers among proteins or a ganglioside extracted were dramatically increased in RIPA-insoluble fraction by ULTRARIPA kit.

LDH assay of proteins extracted by ULTRARIPA kit Phosphatase activity assay of proteins extracted by ULTRARIPA kit

Enzyme activity assay of proteins extracted by ULTRARIPA kit

Left : Lactate Dehydrogenase activity of proteins extracted by using 1% Triton X-100, A and B buffer. Equivalent enzyme activity are obtained when using A and B buffer.

Right: Total protein phosphatase activity. Protein extracts from RIPA-insoluble fraction of the mouse whole brain by ULTRARIPA® kit B-buffer, RIPA, and 2% SDS buffer were applied to total protein phosphatase assay. Although 2% SDS buffer completely extracted proteins, but it disrupted phosphatases. In contrast, ULTRARIPA® kit showed greatly higher activity of protein phosphatases than 2% SDS and RIPA buffer

Supplier Product Code Product Name Size Price
BDLバイオダイナミクス
BioDynamics Laboratory Inc.(株式会社バイオダイナミクス研究所)

Datasheet

F015

ULTRARIPA kit for Lipid Raft

1kit

$120
Supplier Product Code Product Name Size Price
BDLバイオダイナミクス
BioDynamics Laboratory Inc.(株式会社バイオダイナミクス研究所)

F015

ULTRARIPA kit for Lipid Raft

1kit

$120
Description

UltraRIPA kit, can efficiently and rapidly extract membrane proteins or membrane-associated proteins enriched in lipid rafts with native structure and function.

Storage4°CCAS
LinkULTRARIPA kit for Lipid Raft

Contact

  • mailContact

※Prices are for USA / Canada customers. Prices do not include shipping and handling charges, VAT, import tariffs and service charge etc. 

※Please note that Product Information or Price may be changed without notice.

page top