|Prolyl 4-Hydroxylated Human α-Fibrinogen ELISA Kit
||Assay Range : 2.73 - 175 ng/mL
Sample : EDTA-Plasma, Culture Supernatant
Fibrinogen is one of major component of plasma, and is a hexamer composed of pairs of three polypeptides (α-, β- and γ-chain). Prolyl hydroxylation is known to be essential for folding, secretion, and stability of collagen triple helix.
In the powerful study using 2-Dimensional Image Converted Analysis of Liquid chromatography and mass spectrometry (2DICAL), it is found that proline 565 and 530 residues in α-fibrinogen is hydroxylated by the Prolyl hydroxylase P4HA1. In addition, the plasma level of prolyl 4-hydroxylated α-fibrinogen (HP-FGA) was increased in individuals who suffer pancreatic cancer. The significant difference between the said group (n=43) and healthy subjects (n=43) was observed in the 2-DICAL analysis.