Functional: Human TLR5 Stable Cell Line [NBP2-26269] - Functional analysis of the TLR5 stable cell line. The assay was performed using the NF-kB SEAPorter? Assay Kit (NBP2-25286). The Vector control stable cell line (NBP2-26259) and TLR5 stable cell line (NBP2-26269) were transfected with NF-kB/SEAP reporter plasmid for 16 h. Cells were stimulated with Flagellin (IMG-2205) for 24 h followed by SEAP assay.