Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys18] Antibody [NB21-1142] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1142 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.