GNT graphics

GTX628547-01 WB Image
GST antibody immunoprecipitates GST-fused protein in IP experiments.
IP Sample: GST-fused protein expressed E. coli lysate
A. total lysate
B. Control with 1 ug of preimmune goat IgG
C. Immunoprecipitation of GST-fused protein by 1 ug of GST antibody (GTX26633)
12% SDS-PAGE
The immunoprecipitated GST-fuesd protein was detected by GST antibody (GTX26633) diluted at 1:10000.
EasyBlot anti-goat IgG (GTX628547-01) was used as a secondary reagent.

GNT graphics

GTX628547-01 WB Image
EasyBlot anti-Goat IgG (HRP)
Comparison of western blot analysis with traditional secondary antibody (GTX228416-01 diluted at 1:5000) (1) and EasyBlot anti-Goat IgG kit (GTX628547-01 diluted at 1:2000) (2).
A. 0.2 ug native goat IgG
B. 0.1 ug native goat IgG
C. 1 ug denatured goat IgG
D. 2 ug denatured goat IgG
E. 4 ug denatured goat IgG
F. 8 ug denatured goat IgG