GTX60902 WB Image
Western Blot of (1) acid extracts of HeLa cells treated withsodium butyrate and (2) recombinant histone H3.3, usingRM179 at 1 ug/mL, showed a band of histone H3 acetylatedat Lysine 56 in treated HeLa.

GTX60902 ELISA Image
RM179 specifically reacts to Histone H3 acetylated at Lysine 56(K56ac). No cross reactivity with unmodified Lysine 56 (K56 ctrl),acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac),Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.

GTX60902 ICC/IF Image
Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys56) Rabbit mAb RM179 (red). Actin filaments have been labeled with fluorescein phalloidin (green).