GTX60886 WB Image
Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using RM166 at 0.5 ug/mL, showed a band of histone H3 acetylated at Lysine 18 in treated HeLa.

GTX60886 ELISA Image
Sandwich ELISA against acetylated histone H3 at Lys 18 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using anti-H3CT (RM188, 1 ug/mL) as the capture antibody and biotinylated anti-H3K18C (RM166, 2 ug/mL) as the detection antibody.

GTX60886 ChIP assay Image
ChIP performed on HeLa cells with or without Sodium Butyrate treatment, using H3K18Ac antibody (RM166, 5ug). Real-time PCR was performed using primers specific to the geneindicated.

GTX60886 ELISA Image
RM166 specifically reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3.

GTX60886 ICC/IF Image
Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys18) Rabbit mAb RM166(red). Actin filaments have been labeled with fluorescein phalloidin (green).