GTX60854 WB Image
Western blot of nonreduced(-) and reduced(+) mouse IgG2aƒÈ (20 ng/lane), using 0.2ug/mL of clone RM107. This antibody only reacted to nonreduced Mouse IgG2aƒÈ.

GTX60854 ELISA Image
A titer ELISA of mouse IgG2aƒÈ. The plate was coated with different amounts of mouse IgG2aƒÈ. A serial dilution of RM107 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

GTX60854 ELISA Image
ELISA of mouse immunoglobulins shows RM107 reacted to the Fab region of mouse IgG2aƒÈ;no cross reactivity with IgG2aƒÉ, IgG1, IgG3, IgM, IgA, IgE, human IgG,rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM107 was used as the primary antibody. An alkaline phosphatase conjugated antirabbit IgG as the secondary antibody.