GTX50898 IP Image
Histone H3 (acetyl Lys9) antibody [MABI0305] immunoprecipitates Histone H3 (acetyl Lys9) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 ug whole cell lysate/extract of Histone H3 (acetyl Lys9) protein expressing HeLa cells (0.4uM TSA treatment for 18hr). B : Control with 2.5 ug of pre-immune mouse IgG C : Immunoprecipitation of Histone H3 (acetyl Lys9) protein by 2.5 ug of Histone H3 (acetyl Lys9) antibody [MABI0305] (GTX50898) 12% SDS-PAGE The immunoprecipitated Histone H3 (acetyl Lys9) protein was detected by Histone H3 (acetyl Lys9) antibody [MABI0305] (GTX50898) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221667-01) was used as a secondary reagent.
GTX50898 WB Image
Histone H3 (acetyl Lys9) antibody [MABI0305] detects Histone H3 (acetyl Lys9) protein by Western blot analysis.
A. 20 ug HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr)
B. 20 ug HeLa whole cell lysate/extract (0.4 uM Trichostatin A treatment for 18 hr)
15 % SDS-PAGE
Histone H3 (acetyl Lys9) antibody [MABI0305] (GTX50898) dilution: 1:1000
 antibody [MABI0305] detects Histone H3 (acetyl Lys9) protein at nucleus by immunofluorescent analysis.<br>
Sample: HeLa cells, 0.4M TSA-treated for 18hr, were fixed in 4% paraformaldehyde at RT for 15 min.<br>
Green: Histone H3 (acetyl Lys9) protein stained by Histone H3 (acetyl Lys9) antibody [MABI0305] (GTX50898) diluted at 1:1000.<br>
Red: alpha Tubulin antibody (GTX102078) diluted at 1:500.<br>
Blue: Hoechst 33342 staining.<br> )
GTX50898_ChIP.jpg
GTX50898_1101_ChIP.jpg
 antibody [MABI0305])
 and 5 ug of either control mouse IgG or anti-Histone H3K9ac (acetyl Lys9) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or ƒÀ-globin promoter.)