GTX127344 IP Image
Immunoprecipitation of HMGB1 protein from 293T whole cell extracts using 5 ug of HMGB1 antibody (GTX127344).
Western blot analysis was performed using HMGB1 antibody (GTX127344).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX127344 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using HMGB1(GTX127344) antibody at 1:500 dilution.

GTX127344 WB Image
HMGB1 antibody detects HMGB1 protein by Western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 ug A431 whole cell lysate/extract
C. 30 ug HeLa whole cell lysate/extract
D. 30 ug HepG2 whole cell lysate/extract
12 % SDS-PAGE
HMGB1 antibody (GTX127344) dilution: 1:1000

GTX127344 ICC/IF Image
HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (GTX127344) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.

GTX127344 IHC-P Image
HMGB1 antibody detects HMGB1 protein at nucleus on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
HMGB1 antibody (GTX127344) dilution: 1:500.

GTX127344 IHC-P Image
HMGB1 antibody detects HMGB1 protein at nucleus on mouse vein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse vein.
HMGB1 antibody (GTX127344) dilution: 1:500.

GTX127344 ICC/IF Image
HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody (GTX127344) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

GTX127344 WB Image
Non-transfected (?) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX127344) diluted at 1:5000.

GTX127344 IHC-Fr Image
HMGB1 antibody detects HMGB1 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: HMGB1 stained by HMGB1 antibody (GTX127344) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX127344 WB Image
Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX127344) diluted at 1:500.

GTX127344 WB Image
Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX127344) diluted at 1:500.