GTX121677 ChIP assay Image
Cross-linked ChIP was performed with HepG2 chromatin extract and 5 ug of either normal rabbit IgG or anti-Histone H3K9me3 (trimethyl Lys9) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.

GTX121677 WB Image
Histone H3 (tri-Methyl Lys9) antibody detects Histone H3 (tri-Methyl Lys9) protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (tri-Methyl Lys9) antibody (GTX121677) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX121677 IP Image
Immunoprecipitation of Histone H3 (tri-Methyl Lys9) protein from 293T whole cell extracts using 5 ug of Histone H3 (tri-Methyl Lys9) (GTX121677).
Western blot analysis was performed using Histone H3 (tri-Methyl Lys9) (GTX121677).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX121677 ICC/IF Image
Histone H3K9me3 (trimethyl Lys9) antibody detects Histone H3K9me3 (trimethyl Lys9) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3K9me3 (trimethyl Lys9) protein stained by Histone H3K9me3 (trimethyl Lys9) antibody (GTX121677) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

GTX121677 IHC-P Image
Histone H3K9me3 (trimethyl Lys9) antibody detects Histone H3K9me3 (trimethyl Lys9) protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H3K9me3 (trimethyl Lys9) antibody (GTX121677) diluted at 1:500.

GTX121677 IHC-P Image
Histone H3K9me3 (trimethyl Lys9) antibody detects Histone H3K9me3 (trimethyl Lys9) protein at nucleus in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Histone H3K9me3 (trimethyl Lys9) antibody (GTX121677) diluted at 1:500.