GTX114008 IP Image
Bmi1 antibody immunoprecipitates Bmi1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of Bmi1 protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of Bmi1 protein by 2.5 ug of Bmi1 antibody (GTX114008) 10% SDS-PAGE The immunoprecipitated Bmi1 protein was detected by Bmi1 antibody (GTX114008) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX114008 WB Image
Bmi1 antibody detects BMI1 protein by western blot analysis.
A. 30 ug THP-1 whole cell lysate/extract
B. 30 ug HL-60 whole cell lysate/extract
10% SDS-PAGE
Bmi1 antibody (GTX114008) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114008 ICC/IF Image
BMI1 antibody [N1C3-2] detects BMI1 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde for 15 min.
Green: BMI1 protein stained by BMI1 antibody (GTX114008) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

GTX114008 WB Image
Bmi1 antibody detects BMI1 protein by western blot analysis.
A. 30 ug C8D30 whole cell lysate/extract
B. 30 ug BCL-1 whole cell lysate/extract
C. 30 ug Raw264.7 whole cell lysate/extract
10% SDS-PAGE
Bmi1 antibody (GTX114008) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114008 IHC-P Image
Bmi1 antibody detects Bmi1 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Bmi1 antibody (GTX114008) dilution: 1:500.

GTX114008 IHC-P Image
Bmi1 antibody detects Bmi1 protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
Bmi1 antibody (GTX114008) dilution: 1:500.

GTX114008 WB Image
Bmi1 antibody detects Bmi1 protein by western blot analysis.
A. 30 ug K562 whole cell lysate/extract
B. 30 ug THP-1 whole cell lysate/extract
10% SDS-PAGE
Bmi1 antibody (GTX114008) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114008 WB Image
Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114008 WB Image
Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.