GTX113617 WB Image
GFP antibody detects GFP protein by western blot analysis. Non-transfected (-) and GFP-transfected (+, ) 293T whole cell extracts (1 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with GFP antibody (GTX113617) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113617 IHC-Wm Image
Immunohistochemical analysis (whole mount) of transgenic zebrafish embryo, using GFP(GTX113617) antibody at 1:200 dilution.

GTX113617 WB Image
IP-WB assay to show that Hice1 (GTX113617) co-immunoprecipitated with other Augmin components HAUS6 (GTX118732), HAUS2 (GTX118734) and HAUS1 (GTX118733) in U2OS cells. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113617 WB Image
GFP antibody detects GFP protein by western blot analysis.
A. 1 ug 293T whole cell extract
B. 1 ug 293T whole cell extract expressing GFP-tagged protein
C. 1 ug 293T whole cell extract expressing RFP-tagged protein
12% SDS-PAGE
GFP antibody (GTX113617) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113617 ICC/IF Image
Immunofluorescence analysis of GFP-transfected HeLa. A:GFP is expressed in the tranfected cell. B: The cell expressing GFP can be detected using rabbit anti-GFP ab ( GTX113617, 1:5000) followed by Alexa Fluor 594 (1:500) goat anti-rabbit IgG. C: Merged with DNA probe, the lower cell with no GFP expressed as a negtive control.

GTX113617 IP Image
GFP was immunoprecipitated from bacterial GFP-expressing lysate using 1 ug of anti-GFP antibody (GTX113617).
The precipitated GFP was detected by GTX113617 diluted at 1:15000.