GTX101098 ChIP assay Image
Cross-linked ChIP was performed with HepG2 chromatin extract and 5 ug of either control rabbit IgG or anti-PPAR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to C3 promotor or NOD1 gene.

GTX101098 IP Image
PPAR alpha antibody immunoprecipitates PPAR alpha protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : Control with 2.5 ug of pre-immune rabbit IgG B : Immunoprecipitation of PPAR alpha by 2.5 ug of PPAR alpha antibody (GTX101098) 10% SDS-PAGE The immunoprecipitated PPAR alpha protein was detected by PPAR alpha antibody (GTX101098) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX101098 WB Image
Sample (30 ug of whole cell lysate)
A: Rat primary hepatocyte
7.5% SDS PAGE
GTX101098 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101098 WB Image
Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PPAR alpha antibody (GTX101098) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101098 WB Image
Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PPAR alpha antibody (GTX101098) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.