View of mixed neuron/glial cultures stained with Chicken polyclonal antibody to Neurofilament Light C-1390-50 (red) and Rabbit polyclonal antibody to Neurofilament Heavy, phosphorylated R-1388-50 (green). The Neurofilament Light (NF-L) protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells. In contrast the phosphorylated Neurofilament Heavy (NF-H) has a much rmore restricted expression pattern, being found only in developed axonal neurofilaments. Since both proteins are found in neurofilaments, the red and green patterns overlap, so that neurofilaments containing NF-L and phosphorylated NF-H appear yellowish. In contrast neurofilaments containing only NF-L appear red.