Detection of Human CLOCK by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells.  Antibodies: Affinity purified rabbit anti-CLOCK antibody A302-618A used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.  CLOCK was also immunoprecipitated by rabbit anti-CLOCK antibody A302-617A, which recognizes an upstream epitope.  Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).